Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats

Figure 9

(a–d) Light photomicrographs of immunofluorescence-stained liver sections for the expression of PCNA. (a) CG showing the expression of red fluorescent PCNA in the nuclei (yellow arrow) of individual hepatocytes (H). The nuclei of cells (white arrow) lining the sinusoids (S) show Hoechst nuclear stain but no PCNA reactivity. (b) LFG showing the expression of red fluorescent PCNA in the nuclei (yellow arrow) of many hepatocytes. (c) MSCs + CCl₄G showing the expression of red fluorescent PCNA (yellow arrow) in the nuclei of multiple hepatocytes. (d) MSCsG showing evident reduction in the expression of red fluorescent PCNA in the nuclei (yellow arrow) of hepatocytes (confocal microscopy, anti-PCNA, Hoechst nuclear stain, mic. mag.: 63×). (e) A morphometric analysis applying manual counting for the mean of hepatocyte nuclei with positive red fluorescence for PCNA. Values represent mean ± SD. Different letters indicate significant statistical differences at . ^aSignificant compared with CG1. ^bSignificant compared with CG2. ^cSignificant compared with CG3. ^dSignificant compared with CG4. ^eSignificant compared with LFG. ^fSignificant compared with MSCs + CCl₄G. It reveals that CCl₄ treatment leads to significant increase in the total cell fluorescence for PCNA which is reduced following BM-MSCs treatment particularly in the MSCs G compared to the MSCs + CCl₄G (with continuous exposure to CCl₄).

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