Research Article
Evaluation of RealStar® Alpha Herpesvirus PCR Kit for Detection of HSV-1, HSV-2, and VZV in Clinical Specimens
Table 5
Imprecision testing of the RealStar® and in-house multiplex real-time PCR assays using HSV-1, HSV-2, and VZV extracts.
| | DNA concentration (copies/reaction) | Intra-assay | Interassay | | No. of positive replicates | Mean Cp ± SD (% coefficient of variation) | Mean Cp ± SD (% coefficient of variation) |
| | RealStar® alpha Herpesvirus PCR assay | | HSV-1 | | | | | 6.04 × 102 | 3 | 31.41 ± 0.12 (0.38) | 31.38 ± 0.26 (0.84) | | 6.04 × 101 | 3 | 33.99 ± 0.32 (0.93) | 34.16 ± 0.36 (1.06) | | HSV-2 | | | | | 1.76 × 103 | 3 | 30.90 ± 0.08 (0.26) | 30.71 ± 0.26 (0.80) | | 1.76 × 102 | 3 | 32.98 ± 0.08 (0.25) | 33.37 ± 0.44 (1.30) | | VZV | | | | | 2.29 × 103 | 3 | 31.54 ± 0.13 (0.41) | 31.84 ± 0.35 (1.11) | | 2.29 × 102 | 3 | 34.42 ± 0.39 (1.12) | 34.91 ± 0.68 (1.94) |
| | In-house multiplex PCR | | HSV-1 | | | | | 6.04 × 103 | 3 | 30.79 ± 0.12 (0.39) | 30.80 ± 0.10 (0.33) | | 6.04 × 102 | 3 | 34.09 ± 0.15 (0.45) | 34.09 ± 0.18 (0.52) | | 6.04 × 101 | 3 | 37.46 ± 0.85 (2.28) | 37.84 ± 0.85 (2.26) | | HSV-2 | | | | | 1.76 × 104 | 3 | 30.57 ± 0.16 (0.52) | 30.39 ± 0.23 (0.76) | | 1.76 × 103 | 3 | 34.65 ± 0.21 (0.61) | 34.20 ± 0.51 (1.50) | | 1.76 × 102 | 3 | 37.79 ± 0.57 (1.52) | 37.63 ± 0.56 (1.50) | | VZV | | | | | 2.29 × 104 | 3 | 30.64 ± 0.06 (0.18) | 30.11 ± 0.58 (1.91) | | 2.29 × 103 | 3 | 33.87 ± 0.36 (1.05) | 33.14 ± 0.83 (2.50) | | 2.29 × 102 | 3 | 36.09 ± 0.45 (1.25) | 36.13 ± 0.40 (1.11) |
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