Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition
Figure 2
Effects of TXL on MF of rat after AMI. (a) Myocardial fibrosis was detected by Masson trichrome staining. Image Pro Plus was performed to measure fibrotic fraction, which was calculated as the ratio of the area of fibrosis (stained in red) to the total area of fibrosis and normal cardiomyocytes area (stained in red). Scale bar: 50μm. (b) Effects of TXL on myocardial collagen areas. P<0.01 vs. control group; ##P<0.01 vs. Model group; &&P <0.01 vs. benazepril group. (c) Effects of TXL on the protein expressions of MMP-2, MMP-9, types I and III collagen in the MF were determined by Western blotting analysis. (c1)–(c4) showed corresponding column chart. n=3, P<0.01 vs. control group; ##P<0.01 vs. Model group; &P <0.05 &&P <0.01 vs. benazepril group.