BioMed Research International / 2019 / Article / Tab 3 / Research Article
A High Throughput HPLC-MS/MS Method for Antihypertensive Drugs Determination in Plasma and Its Application on Pharmacokinetic Interaction Study with Shuxuetong Injection in Rats Table 3 Precision and accuracy of the analytes in rat plasma (n = 6).
Compounds Spiked concentration (ng/mL) Intraday Interday Measured (ng/mL) RE (%) RSD (%) Measured (ng/mL) RE (%) RSD (%) metoprolol tartrate 20 21.16 ± 0.91 5.8 4.3 21.16 ± 0.64 5.8 3.0 400 431.98 ± 10.65 8.0 2.5 427.53 ± 12.12 6.9 2.8 4000 3725.53 ± 113.06 -6.9 3.0 3784.38 ± 153.35 -5.4 4.1 hydrochlorothiazide 25 24.07 ± 1.70 -3.7 7.1 26.07 ± 2.37 4.3 9.1 500 531.80 ± 28.55 6.4 5.4 524.21 ± 29.44 4.8 5.6 5000 4551.15 ± 42.31 -9.0 0.9 4510.25 ± 147.90 -9.8 3.3 5 5.27 ± 0.33 5.5 6.2 5.34 ± 0.30 6.9 5.6 nifedipine 100 100.64 ± 2.47 0.6 2.5 106.67 ± 5.20 6.7 4.9 1000 909.20 ± 13.18 -9.1 1.5 902.93 ± 43.30 -9.7 4.8 50 56.37 ± 3.12 12.7 5.5 55.30 ± 1.94 10.6 3.5 valsartan 1000 1074.78 ± 24.16 7.5 2.3 1083.06 ± 23.84 8.3 2.2 10000 8647.62 ± 294.77 -13.5 3.4 8813.82 ± 376.14 -11.9 4.3