A High Throughput HPLC-MS/MS Method for Antihypertensive Drugs Determination in Plasma and Its Application on Pharmacokinetic Interaction Study with Shuxuetong Injection in Rats

Table 4

Extraction recoveries and matrix effects of the analytes (n = 6).

Compounds	Spiked; concentration (ng/mL)	Extraction recovery (%)	RSD; (%)	Matrix effect; (%)	RSD; (%)
metoprolol tartrate	20	99.95 ± 2.66	2.7	99.99 ± 1.98	2.0
	400	91.00 ± 1.92	2.1	90.86 ± 1.44	1.6
	4000	95.36 ± 1.08	1.1	91.77 ± 0.63	0.7
hydrochlorothiazide	25	111.50 ± 7.24	6.5	108.41 ± 7.42	6.9
	500	99.77 ± 3.48	3.5	107.96 ± 3.32	3.1
	5000	98.09 ± 2.84	2.9	98.63 ± 2.72	2.8
	5	92.76 ± 5.36	5.8	67.83 ± 5.34	7.9
nifedipine	100	86.20 ± 2.52	2.9	70.52 ± 0.43	0.6
	1000	89.44 ± 2.44	2.7	82.64 ± 0.38	0.5
	50	111.53 ± 7.45	6.7	97.45 ± 6.73	6.9
valsartan	1000	98.55 ± 4.58	4.6	97.73 ± 4.71	4.8
	10000	100.81 ± 2.27	2.3	90.56 ± 1.22	1.3