BioMed Research International / 2019 / Article / Tab 5 / Research Article
A High Throughput HPLC-MS/MS Method for Antihypertensive Drugs Determination in Plasma and Its Application on Pharmacokinetic Interaction Study with Shuxuetong Injection in Rats Table 5 Stability of the analytes in rat plasma (n = 3).
Compounds Spiked Concentration (ng/mL) room temperature for 4 h three freeze-thaw cycles auto-sampler for 12 h -70°C for 14 days Measured (ng/mL) RSD (%) Measured (ng/mL) RSD (%) Measured (ng/mL) RSD (%) Measured (ng/mL) RSD (%) metoprolol tartrate 20 20.95 ± 0.73 3.5 20.05 ± 1.71 8.5 21.42 ± 0.17 0.8 22.12 ± 1.33 6.0 400 437.09 ± 4.45 1.0 412.18 ± 8.83 2.1 408.30 ± 5.14 1.3 448.14 ± 4.22 0.9 4000 3939.11 ± 62.55 1.6 3941.70 ± 205.62 5.2 3732.32 ± 25.44 0.7 3590.13 ± 57.20 1.6 hydrochlorothiazide 25 25.88 ± 0.34 1.3 26.69 ± 1.29 4.8 25.45 ± 0.29 1.1 26.74 ± 3.13 11.7 500 560.13 ± 0.70 0.1 517.28 ± 11.62 2.3 468.47 ± 1.66 0.4 531.65 ± 21.94 4.1 5000 4719.25 ± 205.46 4.4 4739.30 ± 167.66 3.5 4700.72 ± 23.23 0.5 4745.30 ± 36.49 0.8 5 5.46 ± 0.34 6.3 5.14 ± 0.06 1.2 4.87 ± 0.10 2.0 4.61 ± 0.09 2.0 nifedipine 100 100.30 ± 0.78 0.8 99.39 ± 1.34 1.4 97.56 ± 1.09 1.1 97.93 ± 1.18 1.2 1000 873.99 ± 21.28 2.4 928.74 ± 16.08 1.7 927.25 ± 3.49 0.4 860.37 ± 22.96 2.7 50 54.29 ± 0.94 1.7 49.32 ± 2.03 4.1 48.57 ± 1.40 2.9 52.12 ± 3.14 6.0 valsartan 1000 937.54 ± 19.41 2.1 901.96 ± 6.34 0.7 990.30 ± 7.76 0.8 1066.59 ± 68.31 6.4 10000 9192.99 ± 167.34 1.8 9957.94 ± 963.19 9.7 8701.16 ± 60.77 0.7 8719.00 ± 100.69 1.2