Calcium Oscillatory Behavior and Its Possible Role during Wound Healing in Bovine Corneal Endothelial Cells in Culture

Figure 1

Characterization of the cellular calcium response during the FCW. (a-c) Delimitation of the individual cells and classification of the cells into successive rows (see Materials and Methods). (d) Examples of the dynamic behavior of different cells and (e) graphical indication of the signal parameters analyzed in this study. The short peak at the left corresponds to the needle reflection during wounding and sets time zero for the measurements. RFI: relative fluorescence intensity (fold increase over basal value), TFP: time to achieve the maximum fluorescence intensity value of the first peak, and Amp: maximum RFI of the first peak. The arrows indicate the different signal peaks. NP is the total number of peaks in the corresponding trace. For the example shown in (e): row number = 7, TFP = 7s, Amp = 1.69, and NP = 3. We consider the mean cell diameter D to be 35 μm. Therefore V = 35 μm/s (V = row x D/TFP). (f) Dynamic responses of the cells of the first row and (g) average RFI values of the different rows. Bar = 300 μm.

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