Calcium Oscillatory Behavior and Its Possible Role during Wound Healing in Bovine Corneal Endothelial Cells in Culture

Figure 3

Average row parameters in double-wounding experiments. For the double-wounding experiments (a), a first wound (old wound, OW) was performed. A second wound (new wound, NW) was made 10 to 20 min later, to assure complete extinction of the first FCW. In the figure, 1, 2, and 3 designate the wound borders considered for the measurements. The asterisk () indicates the direction of the wave to the uninjured tissue. The plots of TFP (b), Amp (c), NP (d), and V (e) vs the row number show that the curves maintain their characteristics beyond the OW, revealing that the tissue gap has not precluded the propagation and properties of the wave triggered by NW. , and : fast calcium waves triggered by NW between borders 1 and 2 and between border 3 and the uninjured tissue (), respectively. Control parameter values were determined in an independent coverslip. Bar = 200 μm. The figure shows results obtained from typical experiments with total numbers of analyzed cells equal to 465 (control) and 425 (double wounding experiment).

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