LncRNA-TWIST1 Promoted Osteogenic Differentiation Both in PPDLSCs and in HPDLSCs by Inhibiting TWIST1 Expression
Figure 2
The expression level of lncRNA-TWIST1 increased in PPDLSCs during osteogenic differentiation. (a) The expression levels of lncRNA-TWIST1 at different osteogenic time points in PPDLSCs (P< 0.05, P< 0.01). (b) LncRNA-TWIST1 was highly correlated with the Runx2 during osteogenic differentiation (R2 = 0.9489). (c) PDLSCs transfected by an lncRNA-TWIST1-overexpressing lentivirus were observed under a fluorescence microscope (×400). (d) The efficiency of an lncRNA-TWIST1-overexpressing lentivirus was tested with real-time PCR (P<0.05). (e) PDLSCs transfected with an lncRNA-TWIST1-knockdown lentivirus were observed under a fluorescence microscope (×400). (f) The efficiency of the lncRNA-TWIST1-knockdown lentivirus was tested with real-time PCR (P<0.05). Mean ± SEM.