Molecular Cloning and Expression Analysis of Interleukin-8 and -10 in Yellow Catfish and in Response to Bacterial Pathogen Infection

Figure 6

Clostridium butyricum treatment differently regulated the expression of yellow catfish IL-8 (a) and IL-10 (b) challenged by Aeromonas punctata. Fifteen-day-old yellow catfish were equally divided into two groups and fed with a standard commercial diet mixed with or without 2×10⁸ CFU/g Clostridium butyricum for 30 days. Fish were then exposed to Aeromonas punctata infection by bath infection for 24, 48, and 96 h, followed by RT-PCR analysis of the gene expressions of IL-8 and IL-10. C, control group; CB, Clostridium butyricum treated group. 18S gene served as the internal reference. Results were represented as mean ± SEM. : P<0.05; : P<0.01; : P<0.001.

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