Obesity-Induced Upregulation of ZBTB7A Promotes Lipid Accumulation through SREBP1

Figure 4

ZBTB7A was induced in vitro. (a) ZBTB7A expression was evaluated in LO2 normal liver cells and liver cancer cell lines. (b) ZBTB7A protein expression in several cell lines was analyzed with semiquantitative statistics with Quantity One software. (c) HepG2 cells were treated with oleic acid at the concentration of 1 μmol/L, and with another culture for 24 h, the cells were stained with Oil Red reagent and costained with crystal violet to display the cell nucleus. (d–f) The HepG2 cells were treated with 1 μmol/L oleic acid for 24 h, and qRT-PCR was subjected to detect the mRNA levels of ZBTB7A, SREBP1, and Fas1. (g) HepG2 cells were treated with different doses of oleic acid, and western blotting was used to reveal the protein levels of ZBTB7A and SREBP-1c. The SREBP-1c antibody could recognize the precursor and mature forms of SREBP-1c. (h) The protein expression of ZBTB7A and that of SREBP-1c were quantified with a semiquantitative statistic, and the right panel shows the Pearson correlation coefficient between ZBTB7A and mature SREBP-1c expression.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)