Obesity-Induced Upregulation of ZBTB7A Promotes Lipid Accumulation through SREBP1

Figure 5

ZBTB7A increased SREBP-1c expression and activated the activity of NF-κB. (a) LO2 cells and (b) HepG2 cells were transfected with ZBTB7A and vector for 24 h. The mRNA levels of ZBTB7A were examined with qRT-PCR (n = 3). (c) LO2 cells and (d) HepG2 cells were transfected with the pGL3-SREBP1 reporter plasmid and ZBTB7A or vector after transfected with 24 h, and luciferase reporter activity was assessed (n = 4). (e-f) LO2 and HepG2 cells were stably transfected with Luc-NF-κB and then transfected with ZBTB7A and vector; then, the luciferase reporter activity was subjected to the luciferase reporter kit (n = 4). (g) The left panel shows the putative ZBTB7A binding sites within human SREBP1. And the right panel shows the ChIP assay in HepG2 cells with ZBTB7A antibody, and the IgG was used as a negative control.

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