Obesity-Induced Upregulation of ZBTB7A Promotes Lipid Accumulation through SREBP1

Figure 7

Knocking down the expression of ZBTB7A suppressed lipid accumulation induced by Oil acid in HepG2 cells. (a) Knocking down the expression with shRNA with a hairpin, HepG2 cells were transfected for 24 h, and western blotting was subjected to assess the efficiency of knockdown. (b) HepG2 cells were transfected with ZBTB7A knockdown plasmid for 24 h, and the cells were treated with oleic acid for 24 h and then subjected to the Oil Red staining and costained with crystal violet to display the cell nucleus. (c) The same procedure was applied as previously indicated, and then the cells were dissolved in ethanol, and the lipid droplets were dissolved in the solution and then subjected to microplate reader at 450 nm (n = 4). (d) The cells were treated as indicated, and the cell medium was subjected to the TG concentration ELISA kit, and the total protein was assessed by BCA methods (n = 3).

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