Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Ambriz-Aviña, Verónica; Contreras-Garduño, Jorge A.; Pedraza-Reyes, Mario

doi:https://doi.org/10.1155/2014/461941

BioMed Research International

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Volume 2014 | Article ID 461941 | https://doi.org/10.1155/2014/461941

Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Verónica Ambriz-Aviña,¹Jorge A. Contreras-Garduño,¹and Mario Pedraza-Reyes¹

Academic Editor: Gerhard Litscher

Received27 Jun 2014

Revised13 Aug 2014

Accepted13 Aug 2014

Published09 Sept 2014

Abstract

Although reports of flow cytometry (FCM) applied to bacterial analysis are increasing, studies of FCM related to human cells still vastly outnumber other reports. However, current advances in FCM combined with a new generation of cellular reporter probes have made this technique suitable for analyzing physiological responses in bacteria. We review how FCM has been applied to characterize distinct physiological conditions in bacteria including responses to antibiotics and other cytotoxic chemicals and physical factors, pathogen-host interactions, cell differentiation during biofilm formation, and the mechanisms governing development pathways such as sporulation. Since FCM is suitable for performing studies at the single-cell level, we describe how this powerful technique has yielded invaluable information about the heterogeneous distribution of differently and even specialized responding cells and how it may help to provide insights about how cell interaction takes place in complex structures, such as those that prevail in bacterial biofilms.

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Copyright © 2014 Verónica Ambriz-Aviña et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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