Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells

Table 2

Effect of treatments on cell viability in differentiated SH-SY5Y cells in the presence of glutamate (20 mM).

Treatment	Percentage cell viability
Control	100.00 ± 0.012
Glutamate (20 mM)	65.80 ± 1.316*
Glutamate + insulin (0.01 μM)	108.01 ± 1.8^##
Glutamate + insulin (0.1 μM)	139.88 ± 10.4^###
Glutamate + insulin (1 μM)	132.89 ± 9.5^###
Glutamate + insulin (10 μM)	128.13 ± 5.4^###

Values are expressed as mean ± SEM of three tests in triplicate. Statistical analysis was done by using one-way ANOVA followed by Tukey’s multiple comparison test. *P < 0.05 compared to control group. ^##P < 0.01 and ^###P < 0.001 compared to glutamate alone.