Peptide Selank Enhances the Effect of Diazepam in Reducing Anxiety in Unpredictable Chronic Mild Stress Conditions in Rats

Table 2

Indicators of the behavior of rats that were not exposed to chronic unpredictable stress at the EPM test before and after the administration of the test substances. 1, time spent in the open arms of the maze, s; 2, time spent in the closed arms of the maze, s; 3, number of hanging events from the open arms; 4, number of squares crossed; 5, number of rears.

Test substance		Saline	Selank	DZ	Selank + DZ
Behavioral measures		Ме; (25%–75%)	Ме; (25%–75%)	Ме; (25%–75%)	Ме; (25%–75%)
Measures of anxiety
1	Before	48,9	40,2	51,4	42,3
		(42,5–109,7)	(16–68,2)	(20–87)	(18–78,4)
	After	10,8	12,2	9	7,8
		(0–30,3)	(0–23,9)	(0–14,5)	(0–16,6)
2	Before	97,5	124,9	99,7	102
		(59,3–109,6)	(96,7–149,2)	(75,9–117,3)	(66,7–132,4)
	After	152,3	147,7	133,7	162,8
		(135,3–167,8)	(127,8–172,2)	(113,3–177,2)	(147,4–170,9)
3	Before	8	7	10	8
		(7–11)	(3–9)	(6–11)	(4–10)
	After	2	5	2	2,5
		(0–6)	(3–9)	(0–6)	(1–4)
Measures of locomotor activity
4	Before	34	36	32	37,5
		(20–49)	(19–42)	(23–53)	(35–42)
	After	14	30	15,5	14,5
		(11–57)	(17–42)	(9–21)	(12–21)
5	Before	8	9,5	7,5	6,5
		(7–9)	(8–13)	(5–10)	(6–10)
	After	5	9,5	6,5	6,5
		(3–10)	(9-10)	(5–8)	(5–8)

Note. Me: median; , comparison of indicators before and after the administration of a course of the test substances (Wilcoxon’s test).