In vivo Study of the Histone Chaperone Activity of Nucleolin by FRAP
Figure 4
Nucleolin depletion does not impact H4-eGFP fluorescence recovery kinetics. (a) Normalized fluorescence recovery (%) of H4-eGFP in the nucleoplasm of control untransfected cells (red, 5 cells) and in cell transfected with siRNA against nucleolin (blue, 3 cells). Each curve represents the fluorescence recovery of a single cell. (b) Normalized fluorescence recovery (%) of H4-eGFP with a faster recovery kinetics: control untransfected cells (red, 2 cells) and siRNA against nucleolin transfected cells (blue, 1 cell). Each curve represents the fluorescence recovery of a single cell. (c) Normalized fluorescence recovery (%) of H4-eGFP with a slower recovery kinetics: control untransfected cells (red, 3 cells) and siRNA against nucleolin transfected cells (blue, 2 cells). Each curve represents the fluorescence recovery of a single cell. (d)/Dissociation rate constant values () of H4-eGFP in control untransfected (red) and nucleolin depleted cells (blue).
