Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning
Figure 5
Electrophoretic mobility of MG-modified HSA treated with isoflurane. HSA was pretreated with isoflurane (0.3 mM) prior to dilution and exposure to MG (3 mM for 7 days at room temperature). Following SDS-PAGE, analysis of Coomassie-stained gels involved visual inspection (a) and densitometry (b) of the major 66 kDa band. Additionally, the minor MG-induced HMW bands (c) were analyzed by integration of densitometric scans using SigmaGel. The densitometric analysis shown in (b) presents the migration changes of the major bands in (a). The migration distance in (c) represents arbitrary units of measurement that originate from the top of the gel. The color tracings are the plus MG samples that were treated (red) and untreated (blue) with isoflurane. The black tracings are the respective minus MG samples.
