The Increased Activity of Liver Lysosomal Lipase in Nonalcoholic Fatty Liver Disease Contributes to the Development of Hepatic Insulin Resistance

Figure 4

The effect of HFD on the DAG production from intracellular TAG in vitro. (a) Homogenates; (b) dense lysosomes; (c) light lysosomes. 10% liver homogenate, dense lysosomal or light lysosomal fractions were incubated 60 min at pH  = 4.5. At the end of incubation, DAG was extracted into chlorophorm-methanol and quantified as described in Section 2. The graph shows the difference between DAG concentration in the sample at the beginning and at the end of the incubation. Open bars  = fasted animals; hatched bars  = fed animals. ⁺𝑃<0.05 fed versus fasted; ^∗𝑃<0.05, ^∗∗𝑃<0.01 HFD fasted versus SD fasted; ^#𝑃<0.05, ^##𝑃<0.01 HFD fed versus SD fed.

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