The Increased Activity of Liver Lysosomal Lipase in Nonalcoholic Fatty Liver Disease Contributes to the Development of Hepatic Insulin Resistance
Figure 6
Alterations of insulin signalling cascade associated with hepatic fat accumulation. All results are expressed as a fold increase in the insulin-stimulated state relative to the basal state. Representative Western blots are shown above each graph. (a) Fold increase in Akt (Ser473) phosphorylation, (b) fold increase in mTOR (Ser2448) phosphorylation. The basal level of protein phosphorylation was determined in the homogenate prepared from the liver of 24 hours fasted animals. The effect of insulin was determined in identically processed samples from animals which had free access to food and 40 min prior to decapitation were administered insulin 6 U/kg. The total protein (Akt or mTOR) expression was determined after striping the membrane and reblotting with anti-Akt or anti-mTOR antibody. Values represent means ± S.E.M. of 7 animals. .
