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Biochemistry Research International
Volume 2012 (2012), Article ID 541906, 8 pages
Association of TCTP with Centrosome and Microtubules
1Department of Developmental Biology & Invertebrate Morphology, Institute of Zoology, Jagiellonian University, 30-387 Krakow, Poland
2CNRS, UMR 6290, Institut de Génétique et Développement de Rennes, Cell Cycle Group, 35043 Rennes, France
3UEB, IFR 140, Faculté de Médecine, Université Rennes 1, 35043 Rennes, France
4Department of Genetics & Evolution, Institute of Zoology, Jagiellonian University, 30-387 Krakow, Poland
5Department of Embryology, Institute of Zoology, University of Warsaw, 02-096 Warszawa, Poland
6Department of Surgery, The Methodist Hospital, Houston, TX 77030, USA
7Immunobiology Laboratory, The Methodist Hospital Research Institute, Houston, TX 77030, USA
Received 16 February 2012; Accepted 6 March 2012
Academic Editor: R. Mark Ghobrial
Copyright © 2012 Mariusz K. Jaglarz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Translationally Controlled Tumour Protein (TCTP) associates with microtubules (MT), however, the details of this association are unknown. Here we analyze the relationship of TCTP with MTs and centrosomes in Xenopus laevis and mammalian cells using immunofluorescence, tagged TCTP expression and immunoelectron microscopy. We show that TCTP associates both with MTs and centrosomes at spindle poles when detected by species-specific antibodies and by Myc-XlTCTP expression in Xenopus and mammalian cells. However, when the antibodies against XlTCTP were used in mammalian cells, TCTP was detected exclusively in the centrosomes. These results suggest that a distinct pool of TCTP may be specific for, and associate with, the centrosomes. Double labelling for TCTP and γ-tubulin with immuno-gold electron microscopy in Xenopus laevis oogonia shows localization of TCTP at the periphery of the γ-tubulin-containing pericentriolar material (PCM) enveloping the centriole. TCTP localizes in the close vicinity of, but not directly on the MTs in Xenopus ovary suggesting that this association requires unidentified linker proteins. Thus, we show for the first time: (1) the association of TCTP with centrosomes, (2) peripheral localization of TCTP in relation to the centriole and the γ-tubulin-containing PCM within the centrosome, and (3) the indirect association of TCTP with MTs.
Translationally Controlled Tumour Protein (TCTP) is implicated in a broad diversity of cellular functions. It stimulates cell proliferation, growth, survival, and stress response . It is very abundant in highly proliferating cells, including cancer cells. The interest in TCTP increased rapidly in recent years because of the growing body of evidence for its key role in carcinogenesis and rare phenomenon of tumour reversion [2, 3]. Recently, it was elegantly demonstrated that TCTP expression is negatively regulated by p53 and vice versa, that is, TCTP negatively regulates p53 cellular levels via induction of its degradation triggered by MDM2 ubiquitin ligase . The evidence of the reciprocal feedback between TCTP and p53 gives additional proof of the importance of TCTP in cancer development and progression/reversion. TCTP is also associated with the cytoskeleton and throughout this association impacts cell shape, motility, metastasis, and the aggressiveness of cancer. It has been established that TCTP associates both with actin microfilaments (MFs) and MTs . Biochemical analysis of these interactions suggested that, most likely, the TCTP interacts with MFs and MTs indirectly; however, details of these interactions remain unknown (ibid.). TCTP knock down modifies drastically the cell shape and both MFs and MTs architecture [5, 6]. TCTP acts in competition with actin-binding protein cofilin . Because the cofilin promotes actin disassembly, the competition with TCTP may result in increased actin polymerization in cells with higher TCTP levels. Much less is known about the relationship between TCTP and MTs. We have shown that TCTP and tubulin localization in Xenopus and human cells are very similar, but not identical suggesting a presence of “TCTP fibers” unrelated to MTs as well as the presence of TCTP-negative MTs . TCTP localization within the mitotic spindle also does not overlap tubulin localization—it has more homogenous pattern, which suggests that either only a subpopulation of TCTP is associated with MTs or that TCTP localizes in the vicinity but not directly on MTs. On the other hand, TCTP seems to be very strongly associated with the poles of the spindle . These observations suggested that TCTP may be associated with MTs via intermediate linker proteins and that TCTP may also be centrosome-associated protein. We investigated these hypotheses in the study presented here.
2. Material and Methods
2.1. Tissue Culture Cells
The XL2 cell line was cultured in L-15 medium supplemented with 10% fetal calf serum (FCS; full medium) and incubated at 25°C in air. HeLa, NIH3T3, and Cos7 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum (FCS) and incubated at 37°C in 5% CO2. Media were supplemented with penicillin (100 Units/mL) and streptomycin (100 mg/mL).
Cells seeded on glass coverslips were fixed in 75% methanol, 3.7% formaldehyde, 0.5x PBS, or in 3.7% paraformaldehyde in 1x PBS for 10 min at room temperature and permeabilized with 0.1% Triton X100 in PBS for 5 min. DNA was visualized using DAPI. Polyclonal antibodies against XlTCTP (produced in the laboratory in Rennes) and against HsTCTP (Santa-Cruz) or rat TCTP were used at the dilution of 1 : 1000 and 1 : 100, respectively, with overnight incubations at 4°C. Anti-α-tubulin (Sigma) and anti-β-tubulin (Euromedex) were diluted 1 : 200. Purified anti-c-myc antibody (Sigma) was diluted 1 : 100. Secondary antibodies (RITC conjugated, 1 : 1000 dilution; Molecular Probes) were incubated for 1 hr at room temperature. Coverslips were mounted in Vectashield and examined using a Leica DMRXA2 fluorescence microscope or Leica Confocal SP2 microscope. Photographs were taken using a black and white COOLsnap ES camera (Roper Scientific), and images were processed using Metamorph software (Universal Imaging).
2.3. Cell-Free Extracts and In Vitro Spindle Assembly
Cytostatic factor-arrested extracts (CSF extracts) were prepared as described by Murray . For in vitro spindle assembly, 0.5 μL of rhodamine-labeled bovine brain tubulin (Cytoskeleton) was added at 0.2 mg/mL and 2 μL of sperm heads at a concentration of ~1000 nuclei/μL added to 50 μL of the extract and incubated for 60–90 min at 21°C. Spindles (15 μL of extract) were prefixed in 1 mL BRB80 buffer (80 mM K-Pipes, pH 6.8, 1 mM EGTA, and 1 mM MgCl2) containing 30% glycerol, 1% paraformaldehyde, and 0.5% Triton X-100 and centrifuged (2300 xg, 30 min at room temperature) through a 40% glycerol cushion in BRB80 onto glass coverslips in 12-well plate. They were fixed by adding 1 mL cold methanol (−20°C) for 10 min at room temperature (isolated spindles). Then fixed spindles were processed for immunocytochemistry for TCTP using anti-XlTCTP, viewed, and photographed as the cells above.
2.4. Cell Transfection
For transfection of XL2 and NIH3T3 cells with plasmids encoding Xenopus Myc-TCTP, cells were plated on glass coverslips in a 12-well plate. Cells were transfected with 0.5 μg of plasmid DNA using FuGENE 6 transfection reagent (ROCHE) following the manufacturer’s instructions.
2.5. Mouse Oocytes
Three-month-old Swiss albino females were injected intraperitoneally with 10 IU pregnant mare serum gonadotrophin (PMSG; Folligon, Intervet, Holland) to stimulate the development of ovarian follicles. Forty eight to fifty two hours later females were killed by cervical dislocation. Fully grown oocytes arrested at prophase of the first meiotic division—germinal vesicle stage (GV) were released from ovarian follicles. Oocytes were freed from cumulus cells by pipetting and then cultured for 2 h in M2 medium containing bovine serum albumin (BSA; 4 mg/mL). Oocytes that resumed meiosis, that is, underwent germinal vesicle breakdown (GVBD) within first 2 h of in vitro culture, were used for further manipulations and collected for the following stages: GVBD, MI, (6 hrs after GVBD), and MII (20 hrs after GVBD). Oocytes were fixed in 3.7% formaldehyde in PBS, permeabilized with 0.01 Triton X100 in PBS, and subjected to immunofluorescence after incubation in the presence of XlTCTP antibody, the same as with the tissue culture cells.
2.6. Xenopus Laevis Tadpole Ovaries and Electron Microscopy
The developing ovaries were removed from anaesthetized tailed and tailless froglets (stages 62–66) of wild-type Xenopus laevis. Ovaries were fixed in TEM fixative (2% formaldehyde, 3% glutaraldehyde, EM grade, Ted Pella, Redding, CA, in 0.1 M sodium cacodylate buffer pH 7.3, Polysciences, Warrington, PA) containing 10 μm taxol (Cytoskeleton, Denver, CO) to stabilize the microtubules and centrioles. To enhance the visualization of centrioles, the material was stained in 0.5% uranyl acetate, and the osmium tetraoxide treatment was omitted. This resulted in very light staining of all membranous structures; however, it allowed the visualization of highly contrasted centrioles and microtubules. Embedding and sectioning were done as described by Kloc et al. . Postembedding immunostaining using anti-XlTCTP and anti-γ-tubulin antibodies was performed as described in Bilinski et al. . For immunogold labeling, the ovaries were fixed as above. Ultrathin sections (60 nm thick) were collected on nickel single-slot grids (coated with formvar) and blocked with 2% bovine serum albumin (BSA; Sigma) in PBS and 0.1% NaN3 for 30 min, after overnight incubation at 4°C with the primary antibodies (rabbit anti-TCTP, or mouse monoclonal anti-γ-tubulin [GTU-88], ab11316, Abcam) diluted 1 : 50−1 : 100 in the incubation solution (PBS, 1% BSA, 0.1% NaN3). Following several washes in PBS, the grids were incubated for two hours, at room temperature, with the secondary antibody (goat anti-rabbit conjugated to 18 nm gold particles or goat anti-mouse conjugated to 10 nm gold particles, Jackson ImmunoResearch Lab.) diluted 1 : 100–1 : 200 in the incubation solution. Subsequently, the grids were washed in PBS and finally in distilled water. After drying, the sections were contrasted with uranyl acetate and lead citrate and viewed with a JEOL 100SX electron microscope at 80 kV. In control experiments, sections were treated exactly the same as described above, but there was no incubation with the primary antibody. The secondary antibodies were also tested for cross-reactivity prior to double labeling experiments.
3. Results and Discussion
We focused our analysis on the localization of TCTP within the mitotic spindle because it allowed us to study simultaneously the association of TCTP with MTs and with centrosomes, which are located at the spindle poles. Immunolocalization of TCTP in mitotic Xenopus laevis XL2 cells clearly showed the presence of TCTP in the mitotic spindle with higher concentration at the spindle poles (Figure 1(a)). The spindle pole accumulation of TCTP was also evident in the spindles isolated from M-phase-arrested cell-free extract (Figure 1(b)). Because in mitotic Xenopus laevis cells and cell-free extract, the TCTP is associated with the spindle poles where the centrosomes are located, this suggests that TCTP may be a centrosomal protein.
TCTP is very evolutionary conserved protein . Thus, we tested antibodies directed against different species TCTP in Xenopus laevis and mammalian cells. Surprisingly, when we used our polyclonal antibody against Xenopus laevis TCTP (XlTCTP) for TCTP detection in murine NIH3T3 and human origin HeLa cells (so called heterologous or interspecies detection), we always observed a very bright staining of centrosomes at the spindle poles (Figures 1(c) and 1(d)). However, when we used homologous antibodies and cells, that is, anti-human TCTP antibody to detect TCTP in human HeLa cells, a uniform staining of the whole spindle was visible (Figure 1(e)), which agreed with our previous study  and studies by Gachet and colleagues . When we used another heterologous combination, that is, the-anti-rat TCTP antibody in monkey Cos7 cells, we also detected clear centrosomal staining (Figure 1(f)). These observations suggest that the subpopulations of immunologically distinct TCTP might be present in the mitotic centrosomes of human and monkey cells, similarly as in Xenopus laevis cells.
To further clarify these observations, we expressed Myc-tagged XlTCTP in Xenopus laevis XL2 cells (homologous expression) and in mouse NIH3T3 cells (heterologous expression) and followed the localization of the recombinant frog protein in these two types of cells via immunofluorescence with anti-myc antibody. Figures 2(a) and 2(b) show examples of anti-Myc immunodetection of exogenous XlTCTP in XL2 cells. In these cells, we always observed MT-associated localization and an accumulation of Myc-tagged XlTCTP around a small negative area at the very tip of the spindle (Figures 2(a) and 2(b)). The control cells expressing Myc tag alone were uniformly stained (Figure 2(c)). In addition, in the interphase XL2 cells, the Myc-XlTCTP was incorporated into distinct cytoplasmic fibers (Figure 2(d)). The Myc-XlTCTP expression in murine NIH3T3 cells resulted in strong localization of TCTP to the spindle poles; however, we have never observed the presence of the TCTP-negative area similar to the one visible in XL2 cells (Figure 1(e)). In the interphase NIH3T3 cells expressing Myc-XlTCTP the frog TCTP was incorporated to the MT-like fibers (Figure 2(f)). These results show that TCTP indeed localizes to the spindle poles both in Xenopus laevis and in mouse cells, but the pattern of its localization is slightly different when homologous and heterologous system of immunodetection is used. Thus, exogenous Myc-XlTCTP is incorporated to the pericentrosomal area in the mitotic XL2 cells, while in the mitotic mouse cells, it is incorporated into the whole mitotic centrosomes. On the other hand, the homogenous immunofluorescence staining of XlTCTP visible in the spindle poles of XL2 cells suggests the presence of XlTCTP within the whole centrosomes. This indicates that, depending on the species or the cell type, the TCTP is localized either at the spindle pole within the centrosome or around the centrosome in the pericentriolar material (PCM) composed of specific proteins (including γ-tubulin).
Mouse oocytes have no centrioles [13, 14], but they have irregular foci of PCM at the spindle poles both in MI and MII phases of meiosis [15–17]. Because mouse oocyte have PCM but do not have centrioles, we used maturing mouse oocytes to analyze whether TCTP associates with the PCM foci. When we stained in vitro maturing mouse oocytes with the anti-XlTCTP, we detected typical images of PCM foci (Figure 3) instead of the whole spindle staining observed when anti-rabbit TCTP antibody was used on mouse oocytes . In GV stage oocytes arrested in prophase of the first meiotic division, a few distinct foci may be detected which are localized mainly next to the oocyte nucleus (called GV for Germinal Vesicle; Figure 3(a)), thus showing the number and pattern of distribution typical to PCM . After GVBD (germinal vesicle breakdown) and during MI and MII, the TCTP-positive foci polarize at the relatively broad spindle poles (Figures 3(b), 3(c), and 3(d)). The same polarization of the PCM foci was shown by Schatten et al. , and Maro et al. . Taken together, these results indicate that the subpopulation of TCTP detected by anti-Xenopus TCTP antibody indeed localizes to the PCM foci.
In contrast to mouse oocytes, Xenopus laevis oogonia (or nest cells) have typical centrosomes formed by centrioles and the PCM . We used these cells to analyze TCTP localization in relation to the MTs and centrosomes using light microscopy immunofluorescence and immunogold electron microscopy detection. Immunofluorescence using anti-β-tubulin and anti-TCTP antibodies in nest cells showed that the distribution of these two proteins was similar to their distribution in XL2 cells, that is, in the majority of cases these two proteins colocalized, but a subpopulation of MTs devoid of TCTP was also detected, and some TCTP-rich areas were devoid of β-tubulin (Figures 4(a) and 4(b); see  for details of similar localization of TCTP and MTs in XL2 cells). Electron microscopy immunogold labeling with the anti-XlTCTP antibody showed that TCTP was always localized at a distance of approximately 24 nm (the diameter of a MT) from the MT, but never directly on the MTs (Figure 4(c)). This indicates that TCTP does not associate with MTs directly, but by some intermediates serving as the linkers. Immunolocalization of β-tubulin and TCTP in mitotic Xenopus laevis oogonia showed that in the metaphase, the whole spindle area (detected with anti-β-tubulin antibody) was heavily stained (Figure 5(a)), while in the telophase, the tubulin-positive midbodies were negative for TCTP (Figure 5(b)) as already shown before in Xenopus laevis XL2 cells . To facilitate identification of centrosomes at the electron microscopy level and to identify precisely the areas of the PCM, we detected anti-γ-tubulin antibody with secondary antibody conjugated with 10 nm gold particles and the anti-XlTCTP antibody with the secondary antibody conjugated with 18 nm gold particles. This double immunostaining showed that γ-tubulin is present in close proximity of the centriole within an irregular PCM cloud, and that TCTP is present in a layer surrounding the PCM (Figure 6, the inset in the bottom right shows schematically the distribution of γ-tubulin and TCTP domains around the centriole labeled with asterisk). Thus, the TCTP associates with the PCM of the centrosome, but it does not colocalize with γ-tubulin.
In conclusion, we show here that TCTP associates with the centrosomes in Xenopus laevis, human, monkey, and mouse cells and with the PCM foci in acentriolar mouse oocytes. Moreover, within the centrosomes, the TCTP associates with the external part of the PMC foci but not directly with the centrioles. We also show that TCTP associates with MTs at a distance of about 24 nm. This strongly suggests that the MT-TCTP association requires linkers, whose nature, at present, remains unknown. Though we still do not know the role of TCTP at the centrosomes, considering the fact that the aberrant duplication of centrosomes is a key factor in carcinogenesis (reviewed by [20, 21]), our observations open a new avenue into the study of TCTP/centrosome interactions. Interestingly, p53 was also shown to be associated with the centrosomes . Taking into account the reciprocal negative feedback between TCTP and p53 , the potential role of TCTP within the centrosome may involve the antagonistic interaction between these two proteins.
The authours would like to thank Ms. Ada Jankowska and Beata Szymanska for excellent technical support and Ms. Elzbieta Kisiel for helping with the figures. M. K. Jaglarz was supported by Grant K/ZDS/001962 and J. Z. Kubiak by Grants from ARC and LCC.
- U.-A. Bommer and B.-J. Thiele, “The translationally controlled tumour protein (TCTP),” The International Journal of Biochemistry & Cell Biology, vol. 36, no. 3, pp. 379–385, 2004.
- M. Tuynder, G. Fiucci, S. Prieur et al., “Translationally controlled tumor protein is a target of tumor reversion,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 43, pp. 15364–15369, 2004.
- A. Telerman and R. Amson, “The molecular programme of tumour reversion: the steps beyond malignant transformation,” Nature Reviews Cancer, vol. 9, no. 3, pp. 206–216, 2009.
- R. Amson, S. Pece, A. Lespagnol, et al., “Reciprocal repression between P53 and TCTP,” Nature Medicine, vol. 18, no. 1, pp. 91–99, 2011.
- F. Bazile, A. Pascal, I. Arnal, C. le Clainche, F. Chesne, and J. Z. Kubiak, “Complex relationship between TCTP, microtubules and actin microfilaments regulates cell shape in normal and cancer cells,” Carcinogenesis, vol. 30, no. 4, pp. 555–565, 2009.
- F. R. Yarm, “Plk phosphorylation regulates the microtubule-stabilizing protein TCTP,” Molecular and Cellular Biology, vol. 22, no. 17, pp. 6209–6221, 2002.
- K. Tsarova, E. G. Yarmola, and M. R. Bubb, “Identification of a cofilin-like actin-binding site on translationally controlled tumor protein (TCTP),” FEBS Letters, vol. 584, no. 23, pp. 4756–4760, 2010.
- A. W. Murray, “Chapter 30 cell cycle extracts,” Methods in Cell Biology, vol. 36, pp. 581–605, 1991.
- M. Kloc, S. Bilinski, M. T. Dougherty, E. M. Brey, and L. D. Etkin, “Formation, architecture and polarity of female germline cyst in Xenopus,” Developmental Biology, vol. 266, no. 1, pp. 43–61, 2004.
- S. M. Bilinski, M. K. Jaglarz, M. T. Dougherty, and M. Kloc, “Electron microscopy, immunostaining, cytoskeleton visualization, in situ hybridization, and three-dimensional reconstruction of Xenopus oocytes,” Methods, vol. 51, no. 1, pp. 11–19, 2010.
- J. Hinojosa-Moya, B. Xoconostle-Cázares, E. Piedra-Ibarra, A. Méndez-Tenorio, W. J. Lucas, and R. Ruiz-Medrano, “Phylogenetic and structural analysis of translationally controlled tumor proteins,” Journal of Molecular Evolution, vol. 66, no. 5, pp. 472–483, 2008.
- Y. Gachet, S. Tournier, M. Lee, A. Lazaris-Karatzas, T. Poulton, and U. A. Bommer, “The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle,” Journal of Cell Science, vol. 112, no. 8, pp. 1257–1271, 1999.
- D. Szollosi, P. Calarco, and R. P. Donahue, “Absence of centrioles in the first and second meiotic spindles of mouse oocytes,” Journal of Cell Science, vol. 11, no. 2, pp. 521–541, 1972.
- C. Gueth-Hallonet, C. Antony, J. Aghion et al., “γ-Tubulin is present in acentriolar MTOCs during early mouse development,” Journal of Cell Science, vol. 105, pp. 157–166, 1993.
- G. Schatten, C. Simerly, and H. Schatten, “Microtubule configurations during fertilization, mitosis, and early development in the mouse and the requirement for egg microtubule-mediated motility during mammalian fertilization,” Proceedings of the National Academy of Sciences of the United States of America, vol. 82, no. 12, pp. 4152–4156, 1985.
- H. Schatten, G. Schatten, D. Mazia, R. Balczon, and C. Simerly, “Behavior of centrosomes during fertilization and cell division in mouse oocytes and in sea urchin eggs,” Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 1, pp. 105–109, 1986.
- B. Maro, M. H. Johnson, M. Webb, and G. Flach, “Mechanism of polar body formation in the mouse oocyte: an interaction between the chromosomes, the cytoskeleton and the plasma membrane,” Journal of Embryology and Experimental Morphology, vol. 92, pp. 11–32, 1986.
- F. Miyara, Z. Han, S. Gao, R. Vassena, and K. E. Latham, “Non-equivalence of embryonic and somatic cell nuclei affecting spindle composition in clones,” Developmental Biology, vol. 289, no. 1, pp. 206–217, 2006.
- Z. Polański, S. Hoffmann, and C. Tsurumi, “Oocyte nucleus controls progression through meiotic maturation,” Developmental Biology, vol. 281, no. 2, pp. 184–195, 2005.
- E. A. Nigg, “Centrosome duplication: of rules and licenses,” Trends in Cell Biology, vol. 17, no. 5, pp. 215–221, 2007.
- J. Y. Chan, “A clinical overview of centrosome amplification in human cancers,” International Journal of Biological Sciences, vol. 7, no. 8, pp. 1122–1144, 2011.
- K. Shinmura, R. A. Bennett, P. Tarapore, and K. Fukasawa, “Direct evidence for the role of centrosomally localized p53 in the regulation of centrosome duplication,” Oncogene, vol. 26, no. 20, pp. 2939–2944, 2007.