Research Article

Impaired Caveolae Function and Upregulation of Alternative Endocytic Pathways Induced by Experimental Modulation of Intersectin-1s Expression in Mouse Lung Endothelium

Table 1

Chronic inhibition of intersectin-1s expression causes the activation of alternative endocytic/transcytotic pathways and partially restores caveolae number.

Endocytic/transcytotic structuresControlITSN-1s siRNA, 72 hITSN-1s siRNA, 24 d

Caveolae open to the lumen*
Caveolae apparently free in the cytosol
Total caveolae (luminal and free in the cytosol)
Abnormal endocytic structures (enlarged endosomes)
Caveolae clusters
Membranous rings
Tubules

*Results are normalized per 100 μm EC length.