Overexpression of PGC-1α Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells
Figure 1
Retrovirus-mediated PGC-1α overexpression in human skeletal muscle cells. Cultured myoblasts were infected 24–48 h post seeding with 100% medium containing positive control virus pBABE-zsGreen in the presence of 2 μg/mL polybrene. Cells were incubated in virus-containing medium for 24 h, as described in Section 2. Images were taken 48 h post infection. (a) Fluorescence microscope image. (b) Light microscope image.