Overexpression of PGC-1α Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells
Figure 4
Effects of PGC-1α overexpression on mRNA expression of genes involved in lipid metabolism (a), mitochondrial function and biogenesis (b) and on protein levels of mitochondrial components cytochrome C and COXIV (c). (a) and (b): mRNA was isolated from cultured myotubes infected with either empty control or PGC-1α eight days after the onset of the differentiation. Expression was assessed by RT-PCR as described in Section 2, and values are presented as means ± SEM of 3 experiments, each representing one donor, with 3 replicates each, normalized to levels of the housekeeping gene 36B4. A fold change ≥2 or ≤0.5 was considered an increase or decrease in expression level, respectively. (c): Aliquotes from total cell lysates were electrophoretically separated and immunoblotted with specific antibodies as described in Section 2. Images represent a single experiment.
