A Critical Appraisal of Quantitative Studies of Protein Degradation in the Framework of Cellular Proteostasis

Table 1

	Type of study		Conclusion
1	□	Untagged protein	Good approach
2	□	Tagged protein	Not a good approach
3	□	Usual steady state	Partial evidence
4	□	Kinetic (time-course)	Partial evidence
5	□	Protein synthesis inhibitor	Partial evidence for pre-existing proteins in the cell
6	□	Radioactive pulse-chase experiment	Partial evidence for newly synthesized proteins
7	□	Inhibitors of protein degradation	Partial evidence
8	□	Tet-on Tet-off, RNA interference	Partial evidence
9	□	SILAC-MS steady-state experiments under basal conditions (turn-over)	Good evidence
10	□	SILAC-MS upon cell stimulus or stress	Partial evidence