Table 1

Type of studyConclusion

1Untagged protein Good approach
2Tagged proteinNot a good approach
3Usual steady statePartial evidence
4Kinetic (time-course)Partial evidence
5Protein synthesis inhibitorPartial evidence for pre-existing proteins in the cell
6Radioactive pulse-chase experimentPartial evidence for newly synthesized proteins
7Inhibitors of protein degradationPartial evidence
8Tet-on Tet-off, RNA interferencePartial evidence
9SILAC-MS steady-state experiments under basal conditions (turn-over)Good evidence
10SILAC-MS upon cell stimulus or stressPartial evidence