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Figure 3: Western blot analysis of detergent-skinned rat cardiac muscle fibers reconstituted with cTnCWT, c T n C L 2 9 Q or c T n C G 1 5 9 D . Muscle protein samples were separated on 12.5% SDS-PAGE. Proteins from gel were transferred to a PVDF membrane and RcTnC was probed with an anti-TnC primary antibody (Fitzgerald M5092922). Protein profiles in lanes 1–5 represent the following: lane 1, purified RcTnC; lane 2, fibers reconstituted with c-myc RcTnT + RcTnI + RcTnC-WT; lane 3, fibers reconstituted with c-myc RcTnT + RcTnI; lane 4, fibers reconstituted with c-myc RcTnT + RcTnI + RcTnC-L29Q; lane 5, fibers reconstituted with c-myc RcTnT + RcTnI + RcTnC-G159D.