Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics

Figure 3

Western blot analysis of detergent-skinned rat cardiac muscle fibers reconstituted with cTnC_WT, cTnC_L29Q or cTnC_G159D. Muscle protein samples were separated on 12.5% SDS-PAGE. Proteins from gel were transferred to a PVDF membrane and RcTnC was probed with an anti-TnC primary antibody (Fitzgerald M5092922). Protein profiles in lanes 1–5 represent the following: lane 1, purified RcTnC; lane 2, fibers reconstituted with c-myc RcTnT + RcTnI + RcTnC-WT; lane 3, fibers reconstituted with c-myc RcTnT + RcTnI; lane 4, fibers reconstituted with c-myc RcTnT + RcTnI + RcTnC-L29Q; lane 5, fibers reconstituted with c-myc RcTnT + RcTnI + RcTnC-G159D.