Figure 5: Comparison of myofilament sensitivity () and cooperativity () of pCa-tension relationships in detergent-skinned fibers reconstituted with cTnC_{WT}, c, or . (a) Effects of RcTnC mutants on (b) Effects of RcTnC mutants on . The Hill equation was fitted to the normalized pCa-tension relationships to estimate and . One-way ANOVA was used to compare and estimates in and fibers with the data from cTnC_{WT} fibers as controls. Values are expressed as mean SEM. Number of fibers tested in each group is as follows: . Minimal statistical significance was set at . ***.