Figure 5: Comparison of myofilament sensitivity () and cooperativity () of pCa-tension relationships in detergent-skinned fibers reconstituted with cTnCWT, c, or . (a) Effects of RcTnC mutants on (b) Effects of RcTnC mutants on . The Hill equation was fitted to the normalized pCa-tension relationships to estimate and . One-way ANOVA was used to compare and estimates in and fibers with the data from cTnCWT fibers as controls. Values are expressed as mean SEM. Number of fibers tested in each group is as follows: . Minimal statistical significance was set at . ***.