824068.fig.006a
(a)
824068.fig.006b
(b)
Figure 6: Comparison of XB distortion rate constant, c, and tension cost, TC, in detergent-skinned fibers reconstituted with c T n C W T , c T n C L 2 9 Q , or c T n C G 1 5 9 D . (a) Effects of RcTnC mutants on c. c was estimated by fitting the R-D model to the force responses from muscle fibers to chirp-length perturbations [35]. (b) Effects of RcTnC mutants on TC. TC was estimated by dividing the maximal ATPase activity with the maximal tension elicited by the muscle fiber. One-way ANOVA was used to compare estimates of 𝑐 and TC in c T n C L 2 9 Q and c T n C G 1 5 9 D fibers with the data from cTnCWT fibers as controls. Values are reported as mean ± SEM. Number of fibers tested in each group is as follows: c T n C W T , 𝑛 = 1 5 ; c T n C L 2 9 Q , 𝑛 = 1 0 ; c T n C G 1 5 9 D , 𝑛 = 1 4 . Minimal statistical significance was set at 𝛼 = 0 . 0 5 , ** 𝑃 < 0 . 0 1 .