Figure 6: Comparison of XB distortion rate constant, c, and tension cost, TC, in detergent-skinned fibers reconstituted with , or . (a) Effects of RcTnC mutants on c. c was estimated by fitting the R-D model to the force responses from muscle fibers to chirp-length perturbations . (b) Effects of RcTnC mutants on TC. TC was estimated by dividing the maximal ATPase activity with the maximal tension elicited by the muscle fiber. One-way ANOVA was used to compare estimates of and TC in and fibers with the data from cTnCWT fibers as controls. Values are reported as mean SEM. Number of fibers tested in each group is as follows: . Minimal statistical significance was set at , **.