Genetic Basis for Variation of Metalloproteinase-Associated Biochemical Activity in Venom of the Mojave Rattlesnake (Crotalus scutulatus scutulatus)

Table 2

Primers used for amplifying metalloproteinase sequences from Crotalus s. scutulatus. Genomic DNA and sequence comparison to other snake venom metalloproteinases (MP). Nonconserved nucleotides are indicated in bold.

Primer	Annealing site	Corresponding sequence
MP (sense)		GCCCCCAAAATGTGTGGGGTAAC
	Atrolysin𝑏 (558–580)	GCCCCCAAAATGTGTGGGGTAAC
	Artolysin𝑐 (567–589)	GCCCCCAAAATGTGTGGGGTAAC
	Atrolysin𝑑 (488–510)	GCCCCCAAAATGTGTGGGGTAAC
	Atrolysin𝑒 (548–570)	GCCCCCAAAATGTGTGGGGTAAC
	Catrocollastatin (537–559)	GCCCTCAAAATGTGTGGGGTAAC
MP (antisense)		TTCTTCTCCCGCCTCCAAAAGTTC
	Atrolysin𝑎 (1329–1306)	TTCTTCTCCCACCTCCAAAAGTTC
	Atrolysin𝑏 (1328–1305)	TTCTTCTCCCGCCTCCAAAAGTTC
	Atrolysin𝑐 (1327–1304)	TTCTTCTCCCGCCTCCAAAAGTTC
	Atrolysin𝑑 (1248–1225)	TTCTTCTCCCGCCTCCAAAAGTTC
	Atrolysin𝑒 (1308–1285)	TTCTATTCCCGCCTCCAAAAGTTC
	Catrocollastatin (1297–1274)	TTCTTCTCCGGCTTCCAAAAGTTC