Biochemistry Research International / 2013 / Article / Tab 1 / Review Article
Acetylcholinesterase Biosensors for Electrochemical Detection of Organophosphorus Compounds: A Review Table 1 OP biosensors based on membrane immobilized AChE.
Mode of detection Transducer Enzyme immobilization method Minimum detection limit Linearity Substrate/enzyme inhibitor Time of incubation (min) Storage stability (days) Reference Potentiometric Nylon and cellulose nitrate/pH electrode Crosslinking 0.038 μ M 0.077 μ M 50 × 103 –2.5 × 103 50 × 103 –2.5 × 103 μ M Trichlorfon, Co-Ral 15 30 15 [23 ] Fiber-optic Glass/sol-gel/polyvinyl Crosslinking 0.53 μ M and 0.023 μ M 0.54–39.8 and 0.022–0.13 μ M Carbaryl, dichlorvos 10 21 [24 ] Amperometric Hybrid mesoporous silica/Pt electrode Entrapment 1.2 × 10−3 μ M 1.0 × 10−3 –0.3 μ M DZN-oxon 15 80 [25 ] Amperometric MWCNTs/PAN/Pt electrode Affinity bonds using concanavalin A 5.0 × 10−9 μ M 3.6 × 10−8 –3.6 × 10−5 μ M Paraoxon 20 120 [26 ] Amperometric PAN/AuNPs/Pt electrode Covalent Bonding 0.026 × 10−5 μ M 3.6 × 10−7 –3.6 × 10−4 μ M Paraoxon 20 30 [27 ] Amperometric Cellophane/AuE Crosslinking 1.45 μ M 1.45–7.26 μ M Paraoxon 15 NR [28 ] Dissolved Oxygen metric Poly (2-hydroxyethyl metacrylate)/oxygen electrode Entrapment 0.119 μ M 0.05–2.62 μ M Aldicarb 5 2 [29 ] Amperometric PVA-SbQ/Pt electrode Entrapment 7.2 × 10−5 , 0.049 μ M NR Paraoxon, thifensulfuron methyl 30 30 [30 ] Amperometric Polyacrylamide/pH electrode Crosslinking 3.62 × 103 μ M NR Dichlorvos 30 50 [31 ] Conductometric Si3 N4 /Ti layer Crosslinking 10 ppb NR Zn+ , Cd+ 30 20 [32 ] Potentiometric Pore glass/H+ electrode Crosslinking 2 × 10−10 M 10−11 –10−4 M OP compounds 30 [33 ] Colorimetric H bond N+ membrane Physical adsorption 1 mg/mL for omethoate, 0.1 mg/mL for dichlorvos, 2 mg/mL for methamidophos, 0.05 mg/mL for chlorpyrifos, 1.5 mg/mL for carbaryl, and 0.8 mg/mL for pirimicarb. Op compounds 15 60 [34 ]
Note: NR: not reported.