Review Article
Acetylcholinesterase Biosensors for Electrochemical Detection of Organophosphorus Compounds: A Review
Table 2
OP biosensors based on nonconducting polymer immobilized AChE.
| Mode of detection | Transducer | Enzyme immobilization method | Minimum detection limit | Linearity | Substrate/enzyme inhibitor | Time of incubation (min) | Storage stability (days) | Reference |
| Amperometric | MWCNTs/PAN/Pt electrode | Affinity Bonding | 5.0 × 10−9 μM | 3.6 × 10−8–3.6 × 10−5 μM | Paraoxon | 20 | 120 | [26] |
| Amperometric | PAN/AuNPs/Pt electrode | Covalent Bonding | 0.026 × 10−5 μM | 3.6 × 10−7–3.6 × 10−4 μM | Paraoxon | 20 | 30 | [27] |
| Amperometric | MSF/PVA/GCE | Entrapment | 0.2 × 10−3 μM | 0.2 × 10−3–44.8 × 10−3 μM | Monocrotophos | 10 | 30 | [35] |
| Amperometric | PVA/SbQ/SPE | Entrapment | 1.91 × 10−2 μM 1.24 × 10−3 μM | NR | Paraoxon and chlorpyrifos-ethyl oxon | 10 | Nr | [36] |
| Amperometric | PVA/SbQ/Pt Electrode | Entrapment | 7.2 × 10−5, 0.18, and 0.049 μM | NR | Paraoxon, maneb, and thifensulfuron methyl | 30 | 30 | [30] |
| Amperometric | PAMAM-Au/CNTs/GCE | Electrostatic interaction | 4.0 × 10−3 μM | 4.8 × 10−3–9.0 × 10−2 μM | Carbofuran | 9 | 21 | [37] |
| Amperometric | MSF/PVA/GCE | Entrapment | 0.05 ppb (0.2 nM) | 0.05–10 ppb | Monocrotophos | 10 | 30 | [38] |
| Amperometric | Nylon Net | Covalent bonding | 1.3–3.9 ppb | NR | Paraoxon | 30 | 15–20 | [39] |
| Amperometric | PBA/SbQ/Pt Electrode | Crosslinking | 25 ppb–1.5 ppm | NR | Chlorpyrifos | 15 | NR | [40] |
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Note: NR: not reported.
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