O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139

<div>Agarose gel electrophoresis of the amplification specificity of optimized TMPCR (a) and 16S rDNA PCR (b), using representative target and nontarget gDNAs. In lanes 1 to 8, amplification reactions contained V. cholerae O1, V. cholerae O139, V. cholerae NAG I, V. cholerae NAG II, Vibrio parahaemolyticus, Vibrio vulnificus, Escherichia coli, and nuclease-free water, respectively. The expected sizes of specific amplicons (base pairs) were compared to the 100 bp standard ladder marker (lane M).</div>

Biochemistry Research International

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Figure 2

Figure 2: O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139

Figure 2 | O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139 