O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139

Table 2

The unequally reciprocal detection of V. cholerae O1 : O139 by the O serogroup-specific TMPCR.

O1	O139
	0.001	0.01	0.1	1	10	100
100	10^5	10^4	10^3	10^2	10^1	1
10		10^3				10^1
1			10^1			10^2
0.1				10^1		10^3
0.01					10^3	10^4
0.001						10^5

The contents (ng) of purified O1 : O139 gDNAs were used in this assay.