Research Article
O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139
Table 4
Amplification specificity of TMPCR and 16S rDNA-specific PCR.
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The specificity (%) = strains showing a positive amplification of expected amplicon sizes by TMPCR as having no cross-hybridization with negative controls/a total of strains tested, multiplied by 100. bAs for 16S rDNA-specific PCR, no cross-hybridization occurred in reactions containing nuclease-free water or no DNA. cNAG I non-O1/non-O139 growing in semisolid agar in the presence of sucrose and mannose but not arabinose. dNAG II non-O1/non-O139 growing in semisolid agar only supplemented with sucrose. |