Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa

Figure 1

Ricinus Communis Agglutinin I (RCA) binding glycoprotein pattern of bovine caput and cauda epididymal spermatozoa and epididymal fluid. Western blots of Triton X-100-soluble fraction of bovine caput (lane 1) and cauda (lane 3) epididymal spermatozoa and caput (lane 2) and cauda (lane 4) epididymal fluid stained with biotinylated RCA exhibited the presence of an array of intense RCA binding polypeptides in the approximate molecular weight range of 55–38 kDa in addition to several faint RCA binding polypeptides. Among the several RCA stained bands, a doublet of 37-38 kDa polypeptides was present in both caput (lane 1) and cauda (lane 3) sperm. Caput (lane 2) and cauda (lane 4) epididymal fluid displayed several faint RCA stained bands. The amount of proteins loaded in each lane was 20 μg.