Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa
Figure 4
(a) and (b) purified fraction of RCA-agarose column fractionated by two-dimensional polyacrylamide gel electrophoresis stained with Coomassie Brilliant blue R (a) exhibited two distinct spots (38 kDa) with pI of 5.4–5.8. Both spots were excised and identified by proteomic analysis. Western blot of same fraction stained with biotinylated RCA (b) reveals three isoelectric variants of 38 kDa polypeptide in the pH range 5.3 to 5.8. (c) Tryptic peptides of the 38 kDa polypeptide identified by MALDI-TOF-TOF proteomic analysis.
