Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa
Figure 5
(a) Western blot immunostained with TEX101 antibody showing total bovine testicular sperm lysate (lane 1) and the particulate (lane 2) and soluble (lane 3) fractions obtained from testicular spermatozoa (2 × 106 spermatozoa). TEX101 is only associated with the particulate fraction. (b) Immunoblot of plasma membrane fractions of testicular (lane 1), caput epididymal (lane 2), and cauda epididymal (lane 3) spermatozoa stained with anti-TEX101. Each lane contained 10 μg protein. TEX101 is present in all three plasma membrane fractions.
