Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa
Figure 7
(a) Immunoblots of cauda sperm plasma membrane fraction treated with N-glycanase analyzed by reducing SDS-PAGE on 15% gels and immunostained with anti-TEX101. Lane 1 represents the untreated TEX101 polypeptide, and lane 2 displays N-glycanase treated TEX101 polypeptide. Note the reduction in molecular weight (~17 kDa) of the deglycosylated sample. Each lane contains 15 μg protein. (b) Western blot analysis of TEX101 polypeptide treated with PIPLC and immunostained with anti-TEX101. Lanes 1 and 2 display the untreated plasma membranes (control) of pellet and supernatant fractions, respectively. TEX101 is present in the pellet fraction (lane 1). Lanes 3 and 4 exhibit the pellet and the supernatant fractions of PIPLC treated plasma membranes, respectively. Note that there is a complete release of TEX101 polypeptide in the supernatant fraction (lane 4) of PIPLC treated plasma membranes. The amount of plasma membrane proteins employed in the control and PIPLC treated experiments was 15 μg.
