Modulatory Effects of Eschscholzia californica Alkaloids on Recombinant GABA_A Receptors

Table 1

Alkaloid content determined using LC-diode array spectrophotometry in the aerial part of E. californica. The amount of alkaloid is expressed as mg/g of dry weight (mean ± standard deviation ()). Five grams of finely ground plant material was macerated (a) in methanol for 5 days and after SDS-based extraction in chloroform (Soxhlet apparatus) and (b) 10-week extraction in methanol solution from 1.872 kg of dried plant material.

(a)

	Alkaloid (mg/g)
	NMT	PRO	CAL	ALL	ESCH	SA	CHE
CH3OH	0.702 ± 0.159	0.137 ± 0.057	2.413 ± 0.331	0.035 ± 0.016	0.902 ± 0.074	0.041 ± 0.002	0.019 ± 0.003
CHCl3-SDS	0.557 ± 0.138	0.136 ± 0.113	1.951 ± 0.415	0.037 ± 0.010	0.545 ± 0.086	0.040 ± 0.015	0.022 ± 0.100

(b)

Alkaloid	(mg/g)
Protopine (PRO)	0.514 ± 0.038
Californidine (CAL)	12.5 ± 1.8
Allocryptopine (ALL)	0.0120 ± 0.0023
Eschscholtzine (ESCH)	8.700 ± 0.51
Sanguinarine (SA)	0.0191 ± 0.0050
Chelerythrine (CHE)	0.068 ± 0.011
Reticuline (RE)	1.095 ± 0.16
N-Methyllaurotetanine (NMT)	5.68 ± 0.72
Caryachine (CAR)	0.410 ± 0.065