Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry

Table 1

Identification of mature subunits in proteolytically processed proteins of chickpea.

Spot number	Full length deduced amino acid sequence	Subunit identification	MW	pI	Amino acid CoV, %
1	MEE­IVK­RFL­GSE­CFS­STF­IKE­FWD­VMK­WKV­LSR­RLA­EVI­GTK­NTF­CIH­KRE­VLM­DGV­FVI­NGV­NDI­AKR­LDK­KRL­DSK­GGL	Medicago truncatula	9517	9.51	35
2	MAK­LLA­LSL­SFC­FLL­FGT­CFA­LRD­QPQ­QNE­CQL­EHL­NAL­KPD­NRI­KSE­GGL­IET­WNP­SNK­QFA­CAG­VAL­SRA­TLQ­PNS­LLQ­TFL­HQR­SPE­IFI­QQG­NGY­FGM­VFP­GCV­ETF­EEP­RES­EQG­EGS­KFS­DSH­QKV­NRF­REG­DII­AVP­TGV­VFW­MFN­DQD­TPV­IAV­SLI­DTS­SFQ­NQL­DQM­PRR­FYL­AGN­HEQ­EFL­RYQ­QEG­SEE­EEN­EGG­NIF­SGF­KRD­FLE­DAL­NVN­RRI­VNK­LQG­RNE­DEE­KGA­IVK­VKG­GLS­ITT­PPE­KEP­RQK­RGS­RQE­EDE­DED­EKR­QPH­RHS­RQD­EDE­DEK­RQP­HHH­SRG­GSK­SQR­DNG­FEE­TIC­TAR­LHQ­NIG­SSS­SPD­IYN­PQA­GRI­KTV­TSF­DLQ­ALR­FLK­LSA­EFG­SLH­KNA­MFV­PHY­NLN­ANS­ILY­ALK­GRA­RLL­YAL­NCK­GNS­VFD­GEL­EAG­RAL­IVP­QNF­AIA­AKS­LSD­RFS­YVA­FKT­NDR­ALI­NVC­QKK­LLQ­LLS­IWK­EMR­PGS­SSS­TAP­FHF­LFH­PAV­TQT­TKQ­QLD­LVP­NQY­E	Legumin, alpha and beta, subunit precursor of Cicer arietinum	56672	6.20	17
3	MAK­LLA­LSL­SLC­FLL­FSN­SFA­LRE­QSQ­QNE­CQL­ERL­DAL­EPD­NRI­ESE­GGL­IET­WNP­NNR­QFR­CAG­VAL­SRV­TLQ­RNA­LRR­PYY­SNA­PQE­IYI­QQG­NGY­FGV­VFP­GCP­ETF­EEP­QES­EQR­ERR­RYR­DSH­QKV­NRF­REG­DII­AVP­TGN­VLW­MYN­DQD­TPV­IAI­SLT­DTG­SSN­NQL­DQI­PRR­FYL­AGN­QEQ­EFL­RYQ­REQ­GKQ­EQE­NDG­NNI­FSG­FKR­DFL­EDA­LNV­NRH­IVD­RLQ­GRN­EDE­EKG­AIV­KVK­GGL­SII­TPP­ERQ­RGS­RQE­EDE­DEK­EER­QPS­RRR­DES­QKG­ESR­RHG­DNG­LEE­TVC­TAK­LRV­NIG­SSP­SPD­IYN­PQA­GRI­NTV­TSL­DLP­VLR­WLK­LSA­EHG­SLR­KNA­LIV­PHY­NRN­ANS­VIY­ALK­GRA­RLQ­VVN­CNG­NTV­FDG­ELE­AGR­ALT­VPQ­NYA­VAA­KSL­SER­FTY­VAF­KTN­DRD­GIA­RLA­GTS­SVI­NDL­PLD­VVA­ATF­NLQ­RNE­ARQ­LKS­NNP­FKL­LVP­PRE­SEK­RAS­A	Vicia narbonensis	54977	7.00	8
4	MDE­VVA­TMD­ISE­ANE­GYA­SCG­SVI­EMS­RQM­KTT­RVG­ARA­QPW­PAC­PGI­PAV­GRV­GSV­LLF­TAR­MGE­GLC­HMF­TTG­SKA­MEC­GVK­MCL­VGW­PGL­AWD­ELG­RSG­CQF­GLN­HRR­PWV­KAV­LDG­QVS­EEE­DIV­SCL­PKL­QKT­AGS­ASE­VEE­AVK­PAV­KQD­KRL­RSV­KVL­SLV­SNL­SLP­FVF­PLS­LSK­PLQ­QMA­DNE­KGN­KVR­SQD­IGT­SSS­RVN­EAP­DTS­CVA­VVQ­HLI­NQN­KLL­IEI­LQQ­HRM­PIL­NPS­QMQ­PQV­QLE­AVQ­ALT­PQV­SAP­PTS­QKA­PHA­MPH­VDP­KEA­SII­CFM­CDE­QGH­YAR­NCP­QQK­RKA­PMR­TED­EVR­KMI­ITS­TEW­PPP­GMT­KHQ­RRN­SFR­GAQ­QLT­EHL­LAN­GGR­VSG­SED­SDQ­VSS­DDE­DEK­SPQ­GFN­QNK­IEL­KAC­SRC­GEI­GHV­ASS­CAS­TCV­HCE­EDH­PPD­RCP­TNK­ITY­FFC­EGT­DHV­PKD­CQF­SFL­LTK­KMA­NQP­ASS­NGE­KHQ­GNT­NPR­QDH­RFS­LTP­VPG­QRN­RNE­KRK­CRV­RED­ICC­FNC­QGM­GHF­ADK­CPK­PRN­IAA­GTS­VHA­TPC­NQK­LAP­QRI­VIH­ASR­SSP­IAR­VAT­API­PMN­ALP­QGV­NAQ­FQP­QPP­ADK­TGA­SIC­VVP­LEV­PIQ­QLR­NQV­QDE­EPE­CKK­VIV­CYN­CSE­EGH­YSK­NCP­QPR­QNR­PPH­YRQ­FTR­SRH­SNR­IVV­TGA­NAV­PVR­PRV­NQN­P	Oryza sativa (rice)	73339	8.75	3
5	MAK­LLA­LSL­SFC­FLL­FGT­CFA­LRD­QPQ­QNE­QLE­HLN­ALK­PDN­RIK­SEG­GLI­ETW­NPS­NKQ­FAC­AGV­ALS­RAT­LQP­NSL­LQT­FLH­QRS­PEF­IQQ­GNG­YFG­MVF­PGC­VET­FEE­PRE­SEQ­GGS­KFS­DSH­QKV­NRF­REG­DII­AVP­TGV­VFW­MFN­DQD­TPV­IAV­SLI­DTS­SFQ­NQL­DQM­PRR­YLA­GNH­EQE­FLR­YQQ­EGS­EEE­ENE­GGN­IFS­GFK­RDF­LED­ALN­VNR­RIV­NKL­QGR­NED­EEK­GAI­VKV­KGG­LSI­TTP­PEK­EPR­QKR­GSR­QEE­DED­EDE­KRQ­PHR­HSR­QDE­DED­EKR­QPH­HHS­RGG­SKS­QRD­NGF­EET­ICT­ARL­HQN­IGS­SSS­PDI­YNP­QAG­RIK­TVT­SFD­LQA­LRF­LKL­SAE­FGS­LHK­NAM­FVP­HYN­LNA­NSI­LYA­LKG­RAR­LLY­ALN­CKG­NSV­FDG­ELE­AGR­ALI­VPQ­NFA­IAA­KSL­SDR­FSY­VAF­KTN­DRA­LIN­VCQ­KKL­LQL­LSI­WKE­MRP­GSS­SST­APF­HFL­FHP­AVT­QTT­KQQ­LDL­VPN­QYE	Legumin, alpha and beta, subunit precursor of Cicer arietinum	56216	6.20	7
6	ETT­AFN­TTS­RIG­NWS­SAI­SPP­LQT­CGS­FKC­QLP­TRR­GVIV  AD­LRN­SNF­RWR­KAT­TTS­RGN­VAA­EAV­KIP­TSV­PVR­VAR­EL  AQAGYRYLDVRT	Arabidopsis thaliana	10249	11.14	45