Inactivation of Anopheles gambiae Glutathione Transferase ε2 by Epiphyllocoumarin

Figure 2

Electrophoretic analysis of the different fractions of AgGSTε2. Lane 1 contained molecular weight markers. Lane 2 contained the supernatant fraction obtained after lysis, sonication, and centrifugation of E. coli cells. Lane 3 contained the nonbound fraction containing protein that did not bind the affinity chromatographic ligands hexyl GSH/GSH. Lane 4 contained the wash fraction. Lane 5 contains the affinity pool fraction. The concentrate fraction is contained in lane 6 whilst lane 7 contained the dialysate fraction. Lanes 2 and 3 contained numerous bands indicating the presence of unwanted proteins. The single bands shown in lanes 5, 6, and 7 indicate that GSTε2 was purified to homogeneity.