Inactivation of Anopheles gambiae Glutathione Transferase ε2 by Epiphyllocoumarin

Table 1

Summary of the purification of recombinant Anopheles gambiae glutathione-S-transferase epsilon 2.

Sample	Total activity; (units)	Total protein; (mg)	Specific activity; (units mg^−1)	Percentage yield; (%)	Fold purity
Supernatant	225	432	0.52	100	1
Nonbound	135	320	0.42	60	0.8
Wash	2.2	104	0.012	1	0.02
Affinity pool	67	11	6.08	26	11.7
Concentrate	76	3	27	36	51.1
Dialysate	81	1.3	46	36	86.2

The activity of the enzyme is expressed per milligram of total protein (expressed in mol min⁻¹ mg⁻¹). Protein was determined by the method of Lowry et al. [12].