Differences in GPR30 Regulation by Chlorotriazine Herbicides in Human Breast Cells

Figure 3

Change in GPR30 expression in MDA-MB-231 cells after atrazine exposure. MDA-MB-231 human breast cancer cells were treated with 0.1- to 10-fold concentrations of the EPA safe levels of atrazine, cyanazine, or simazine for 24 hours. mRNA was harvested, cDNA was synthesized, and specific amplicons were prepared using primers to GAPDH (reference gene) or GPR30. Resulting fold changes in expression ± standard deviation are shown, with the vehicle control set at 1. Statistically significant changes in induction compared to the control are indicated ().