Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice

Figure 1

Cytotoxic effect of para-phenylenediamine and ortho-phenylenediamine (o-PD) on melanoma cells. A375 (a) and B16-F10 (b) cells grown in a 96-well (5000 cells in each well) plate were treated with p-PD of indicated concentrations for 16, 24, and 48 hours at 37°C. (c) In a similar manner, A375 cells were also treated with o-PD for 24 and 48 hours with indicated concentrations. Four wells were used for each concentration in every time point. One set of untreated cells (0.1% DMSO) was kept as control for each time point. The absorbance reading (OD) for each set obtained from the microplate reader was used to compute the percent change in viable cell in treated sets over respective controls using the expression . The % viable cell over control was plotted against respective concentrations of p-PD. Error bars represent SD; .

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