Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice

Figure 2

Body weight gain of the mice treated with or without p-PD. (a) Effect of p-PD on tumour development in mice. Initially, right flanks of two sets of Swiss-Albino mice were given subcutaneous injection with only PBS (A) and B16-F10 cells (B) as mentioned in the Materials and Methods. These mice were kept for 2 weeks until visible tumour in the set (B) is formed. The mice from set (B) were divided into three groups: one (C) was treated with 2 and the other (D) was treated with 4 mg/kg of p-PD for 10 days. One (B) set was left untreated as control. Photographs of representative mouse from each set are shown here. Each set comprised 6 mice except for set (B) where initially 18 mice were used for the experiment. (b) Body weights of untreated mice (set (A) above) (■), mice treated with 4 mg/kg of p-PD (⧫), mice having tumour (- - -) (set (B)), and mice having tumour treated with 2 (▲) (set (C)) and 4 (∙) mg/kg (set (D)) of p-PD are plotted against the days of treatment. Arrows indicate the time of peritoneal p-PD administration. Error bars represent SD; . indicates the two-tailed value ≤ 0.018. By conventional criteria, this difference is considered to be statistically significant.

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