Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice

Figure 3

p-PD mediates apoptosis in melanoma cells. (a) A375 cells were treated with p-PD as indicated. After treatment, cells were lysed with protein loading buffer (Materials and Methods). 30 μg of total protein lysate was loaded in each lane and subjected to western blot and probed for activation of PARP. Cells that were not treated with p-PD (0) but with 0.1% DMSO served as control (lanes 1, 5, and 9). These blots are representative of three independent analyses. β-actin is used as a loading control for all time points. (b) Both A375 and B16-F10 cells were treated for 48 hours with indicated amounts of p-PD and were subjected to flow cytometry analysis using Annexin V-FITC assay. DAPI was used as the DNA stain.

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