Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice

Figure 4

Cell cycle arrest at S phase imposed by p-PD. (a) Cell cycle distribution of A375 cells treated with p-PD as indicated was determined by PI staining using flow cytometry. The number of cells in S phase for each set was determined and plotted against indicated concentration of p-PD. Error bars represent SD; . (b) Flow cytometric analysis of BrdU incorporation by A375 cells treated with indicated concentrations of p-PD. The BrdU positive cell cultures at the S phase are gated in pink. Respective percent cell numbers are shown next to the gated cell clusters representing cell cycle phases.

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