Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice

Figure 6

Caspase 8 inhibitor decreases the number of dead A375 cells. Cells were pretreated with the inhibitors of caspase 8 (a), caspase 9 (b), and PAN caspase (c) for two hours and subsequently treated with p-PD for 24 hours. Percent change in viable cells in sets with inhibitor pretreatment was computed as described in the Materials and Methods. The net protective effect () is either the |value of white bar + value of black bar| or the decrease in dead cell number as denoted by ▲. By conventional criteria, this difference is considered to be extremely statistically significant. indicates the two-tailed value ≤ 0.005. Each set was performed in triplicate.

(a)

(b)

(c)