Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice

Table 4

Change in the biochemical parameters of oxidative stress induced by 100 µg/mL of p-PD for 24 hours on A375 cells.

Glutathione reductase			Catalase			GSH			GSH : GSSG
p-PD (µg/mL)		% change	p-PD (µg/mL)		% change	p-PD (µg/mL)		% change	p-PD (µg/mL)		% change
Cont.	100		Cont.	100		Cont.	100		Cont.	100
12 ± 1.5	17 ± 0.5	39 ± 8.6	5 ± 0.3	6 ± 0.2	22 ± 2	19.2 ± 0.7	5.9 ± 1.9	70 ± 13	62 ± 26	12 ± 4	80 ± 9

These changes indicate percent decrease of the respective parameters in the treated samples. Each set of data is averaged from three different experiments.
Oxidative stress was measured using the standard parameters such as reduced GSH amount, GSH : GSSG, and activity of glutathione reductase and catalase. Parameters were measured as described in Section 2.5. We used control cells and cells treated for 24 hours with 100 µg/mL of p-PD for these experiments. GSH was expressed as nmol mg⁻¹ protein; catalase and glutathione reductase activities were expressed as nmol min⁻¹ mg⁻¹ protein.