Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice

Table 5

Effect of NAC and GSH pretreatment on ROS generation and apoptosis in A375 cells.

p-PD (µg/mL)	% decrease in ROS		% drop in apoptosis
	NAC (10 mM) pretreatment	GSH (5 mM) pretreatment	NAC (10 mM) pretreatment	GSH (5 mM) pretreatment
0	34 ± 3.5	−6 ± 0.9	0	0
20	31 ± 2.6	34 ± 1.4	63 ± 4.7	41 ± 5.6
40	66 ± 4.5	29 ± 2	47 ± 4	18 ± 0.2
100	25 ± 1.2	6 ± 0.3	41 ± 2.1	44 ± 3

A375 cells were divided into 2 groups: one was pretreated with NAC or GSH for 1 hour and then treated with p-PD for 16 hours and the other was treated with only p-PD. ROS values and apoptotic cell numbers were quantified at the end of p-PD exposure as mentioned in the Materials and Methods. Percentage changes in ROS and apoptosis were calculated as follows: % change = (only p-PD value − respective pretreatment value)/respective pretreatment value  ×  100. Values are representative of three independent experiments.